二氧化硅纳米颗粒致HUVEC内质网应激的机制

Molecular mechanisms of endoplasmic reticulum stress induced by silica nanoparticles in human umbilical vein endothelial cell

摘要:

目的探讨二氧化硅纳米颗粒(SiNP)致人脐静脉内皮细胞(HUVEC)内质网应激(ERS)的分子机制。 方法用25、50、100 mg/L SiNP作用于HUVEC,蛋白质免疫印迹、免疫荧光技术检测未折叠蛋白反应(UPR)及其关键蛋白表达。 结果随SiNP作用剂量的增加,HUVEC的UPR及其下游蛋白表达显著上调(P＜0.01)。 结论SiNP可致HUVEC持续ERS,并促进下游关键蛋白表达。

Abstract:

To investigate the possible molecular mechanism of silica nanoparticles (SiNP) induced endoplasmic reticulum stress (ERS) in human umbilical vein endothelial cell (HUVEC). MethodsHUVEC were exposed to 25,50, and 100 mg/L of SiNP, respectively. The expressions of unfolded protein response (UPR) and its downstream key proteins were detected by Western blotting and immunofluorescence techniques . ResultsWith the increasing dose of SiNP, the expression of UPR and its downstream proteins in HUVEC were significantly up-regulated (P＜0.01). ConclusionSiNP can induce sustained endoplasmic reticulum stress in HUVEC and promote the expressions of key downstream proteins.