梅毒螺旋体溶血素生物信息学分析

Bioinformatics analysis of hemolysin family proteins of Treponema pallidum

摘要:

目的本研究旨在对梅毒螺旋体(Tp)的5种溶血素进行进一步的生物信息学分析,为后续研究溶血素家族蛋白在Tp致病过程中的致病机制提供依据。 方法通过使用NCBI、BLAST、CDD、BioEdit、ExPASy、SignalP、TMHMM、MEGA、PSORTb 3.0、ABCpred等生物信息学分析方法分析梅毒螺旋体溶血素家族蛋白的一般性质、信号肽、糖基化、磷酸化位点、亚细胞位置、二级结构、功能结构域及抗原表位。 结果预测了Tp中5个溶血素家族蛋白一般性质,Tp1037功能结构域不同于其余4种,Tp0028含有1个信号肽,Tp0027含有1个糖基化位点,Tp0027和Tp1037有多个跨膜结构域,5个溶血素家族蛋白均含有多个磷酸化位点和B细胞、T细胞表位。 结论Tp含有5个溶血素家族蛋白基因,提示Tp入侵宿主时这些基因产物极有可能通过其膜成孔毒性损伤靶细胞,从而致病。

Abstract:

The purpose of this study was to conduct further bioinformatics analysis of the five hemolysin oftreponema pallidum, and to provide evidence for the pathogenic mechanism of hemolysin family proteins in the pathogenesis oftreponema pallidum. MethodsThe general properties of the syphilis family proteins, signal peptides, glycosylation, phosphorylation sites, subcellular positions, secondary structures, functional domains, and antigen epitopes were analyzed by bioinformatic analysis methods using NCBI, BLAST, CDD, BioEdit, ExPASy, SignalP, TMHMM, MEGA, PSORTb 3.0, ABCpred andother bioinformatics analysis methods. ResultsGeneral properties of five hemolysinfamily proteinsin Treponema pallidum were predicted, Tp1037 functional domains differ from the other four. Tp0028 contains one signal peptide,Tp0027 contains one glycosylation site, all five hemolysin family proteins contain multiple phosphorylation sites and B cell and T cell epitopes. ConclusionTreponema pallidumcontains five hemolysin family protein genes,suggesting that when treponema pallidum invades the host, these gene products are highly likely to undergo their membrane-forming pore toxicity, damage the target cells, and thus cause disease.