白喉乌头对RA大鼠B10细胞和炎症因子的影响

Effects of aconitum diphtheria on B10 cells and inflammatory cytokines in RA rats

摘要:

目的探讨白喉乌头对类风湿关节炎(RA)大鼠B10细胞和炎症因子的影响。方法将40只SPF级雌性Wistar大鼠均分为空白对照组、模型组、低剂量组、高剂量组与阳性对照组。除空白对照组外,其他组大鼠均建立类风湿关节炎模型,造模成功后,模型组生理盐水灌胃,低剂量和高剂量组每天22.5、45 mg/kg白喉乌头灌胃,阳性对照组每天1 mg/kg雷公藤灌胃,每次灌胃1 mL,每天1次,连续4周。HE染色观察各组大鼠踝关节滑膜组织病理学变化,免疫组化法检测各组滑膜组织B10细胞表面抗原CD19、CD5表达情况,ELISA法检测各组大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、IL-1β、IL-10、IL-12的水平。 结果模型组关节滑膜有水肿及大量纤维组织增生；低剂量组、阳性对照组有少量纤维组织增生；高剂量组轻微水肿,无纤维组织增生。模型组CD5、CD19蛋白表达较空白对照组增高(P＜0.05),低剂量组、高剂量组和阳性对照组CD5、CD19蛋白表达较模型组降低(P＜0.05)。模型组TNF-α、IL-6、IL-12、IL-1β水平较空白对照组增加(P＜0.05),低剂量组、高剂量组、阳性对照组TNF-α、IL-6、IL-12、IL-1β水平较模型组降低(P＜0.05)。结论白喉乌头可能通过调控B10细胞减少RA滑膜组织的炎症反应。

Abstract:

To investigate the effects of aconitum diphtheriae on B10 cells and inflammatory cytokines in rheumatoid arthritis (RA) rats. MethodsForty SPF female Wistar rats were divided into blank control group, model group, low-dose group, high-dose group and positive control group. Except for the blank control group, rheumatoid arthritis model was established in other groups. After successful modeling, the model group was intragastric with normal saline, low-dose and high-dose groups were intragastric with 22.5 and 45 mg/kg diphtheria aconitum, positive control group was intragastric with 1 mg/kg Tripterygium wilfordii, 1 mL each time, once a day, for 4 weeks. HE staining was used to observe the histopathological changes of synovial tissues in each group. Immunohistochemical method was used to detect the expression of CD19 and CD5 on B10 cells in synovial tissues of each group. ELISA method was used to detect the serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, IL-10 and IL-12. ResultsThe synovium of the model group had edema and a lot of fibrous tissue hyperplasia. The low dose group and the positive control group had a small amount of fibrous tissue hyperplasia. The high dose group had slight edema and no fibrous hyperplasia. The expressions of CD5 and CD19 protein in model group were higher than those in the blank control group (P＜0.05), and the expressions of CD5 and CD19 protein in low dose group, high dose group and positive control group were lower than those in the model group (P＜0.05). The expressions of TNF-α, IL-6, IL-12 and IL-1β in the model group were increased compared with the blank control group (P＜0.05), The expressions of TNF-α, IL-6, IL-12 and IL-1β in low dose group, high dose group and positive control group were lower than those in the model group (P＜0.05). ConclusionAconite diphtheria may reduce the inflammatory response of RA synovial tissue by regulating B10 cells.