KIAA1456靶向调控Runx1抑制肺癌A549细胞增殖和侵袭

KIAA1456 inhibit proliferation and invasion of non-small cell lung cancer A549 cells by targeting Runx1

摘要:

目的探讨KIAA1456靶向调控Runx1对非小细胞肺癌A549细胞增殖和侵袭的影响。方法采用免疫组织化学法检测91例肺癌及癌旁组织中KIAA1456表达情况,分析KIAA1456与肺癌患者临床病理特征的相关性,并进行Kaplan-Meier生存分析。用Lipofectamine法将miR-NC KIAA1456 RNAi和pCDNA3.0-HA-KIAA1456质粒转染A549细胞,MTT法和Transwell法检测细胞增殖和侵袭能力,Western blotting法检测细胞KIAA1456、Cyclin D1、Runx1和MMP-9表达。结果KIAA1456在肺癌组织中的高表达率明显低于癌旁组织(P＜0.05)；KIAA1456在不同淋巴结转移、TNM分期分层间表达有差异(P＜0.05)。KIAA1456高表达肺癌患者5年总生存率高于KIAA1456低表达者(P＜0.05)。与阴性对照组比较,KIAA1456 RNAi组A549细胞增殖率、侵袭细胞数、Cyclin D1、Runx1和MMP-9表达均增加,KIAA1456表达降低；KIAA1456质粒组A549细胞增殖率、侵袭细胞数、Cyclin D1和MMP-9表达均降低,KIAA1456和Runx1表达增加(P＜0.05)。结论KIAA1456可作为肺癌的抑癌基因,通过靶向调控Runx1表达,抑制A549细胞增殖和侵袭。

Abstract:

To investigate the effect of KIAA1456 targeting Runx1 on the proliferation and invasion of non-small cell lung cancer A549 cells. MethodsImmunohistochemistry was used to detect the expression of KIAA1456 in 91 cases of lung cancer and adjacent tissues, and the correlation between KIAA1456 and the clinicopathological characteristics of lung cancer patients was analyzed, and Kaplan-Meier survival analysis was performed. The miR-NC KIAA1456 RNAi and pCDNA30-HA-KIAA1456 plasmid was transfected into A549 cells by Lipofectamine. The cell proliferation rate and invasion ability were detected by MTT and Transwell. Western blotting was used to detect the expression of KIAA1456, Cyclin D1, Runx1 and MMP-9 in the cells. ResultsThe expression of KIAA1456 in lung cancer tissues was significantly lower than that in the adjacent tissues (P＜0.05). The expression level of KIAA1456 was correlated with lymph node metastasis and TNM stage(P＜0.05).The 5-year overall survival rate of lung cancer patients with high expression of KIAA1456 was significantly higher than that of patients with with low expression of KIAA1456 (P＜0.05). Compared with the negative control group, the proliferation rate, number of invaded cells, Cyclin D1, Runx1 and MMP-9 expression of A549 cells in the KIAA1456 RNAi group were increased, while KIAA1456 expression was decreased, and A549 cell proliferation rate, number of invasion cells, Cyclin D1 and MMP-9 expression in KIAA1456 plasmid group were decreased, KIAA1456 and Runx1 expression were increased(P＜0.05). ConclusionKIAA1456 can act as a tumor suppressor gene in lung cancer. It can inhibit the proliferation and invasion of A549 cells by targeting the expression of Runx1.