低级胶质瘤候选靶标及预后标志物SLC4A家族的生物信息学分析

SLC4A gene family as candidate targets and prognostic biomarkers for low-grade glioma by bioinformatics analysis

摘要:

目的采用生物信息学分析,筛选SLC4A基因家族中可作为低级胶质瘤(LGG)的候选靶标及预后标志物的基因。 方法利用TCGA、GTEx、UCSCXENA、GEPIA、cBioPortal、GeneMANIA、String等数据库,分析LGG患者SLC4A的差异表达、预后价值、遗传改变和蛋白互作等,根据数据筛选SLC4A基因家族中可作为LGG候选靶标及预后标志物的基因。 结果LGG组织中SLC4A2、SLC4A4、SLC4A7、SLC4A8表达升高,而SLC4A1、SLC4A3、SLC4A9、SLC4A10表达降低。SLC4A2、SLC4A3、SLC4A4、SLC4A7、SLC4A10在LGG肿瘤分级中表达存在显著差异。预后价值总生存率的分析发现,高表达SLC4A2、SLC4A3、SLC4A7、SLC4A8和低表达SLC4A5、SLC4A10的患者预后较差；无病生存率的分析发现,高表达SLC4A2、SLC4A3、SLC4A8和低表达SLC4A10的患者预后较差。 结论SLC4A2、SLC4A3、SLC4A7、SLC4A8、SLC4A10可能是LGG潜在候选靶标及预后标志物。

Abstract:

AimTo screen the genes of SLC4A gene family that can be used as candidate targets and prognostic markers in low-grade glioma (LGG) by bioinformatics analysis. MethodsThe differential expression, prognostic value, genetic changes and protein interaction of SLC4A in patients with low-grade gliomas were analyzed, by using TCGA, GTEx, UCSCXENA, GEPIA, cBioPortal, GeneMANIA, String. Based on the data, SLC4A family genes were screened as LGG candidate targets and prognostic markers. ResultsThe results indicated that the expression of SLC4A2, SLC4A4, SLC4A7 and SLC4A8 was increased in LGG tissue, whereas the expression of SLC4A1, SLC4A3, SLC4A9 and SLC4A10 was decreased. SLC4A2, SLC4A3, SLC4A4, SLC4A7 and SLC4A10 were significantly differently expressed with LGG tumor grades. Through the analysis of overall survival (OS) by prognostic value analysis, it was shown that patients with high expression of SLC4A2, SLC4A3, SLC4A7 and SLC4A8, and the low expression of SLC4A5 and SLC4A10, had poor prognosis. In the analysis of disease-free survival (DFS), patients with high expression of SLC4A2, SLC4A3 and SLC4A8, and low expression of SLC4A10, had poor prognosis. ConclusionSLC4A2, SLC4A3, SLC4A7, SLC4A8 and SLC4A10 could be Candidate Targets and Prognostic Biomarkers for LGG patients.