胰岛素样生长因子结合蛋白3在宫内生长受限儿中的甲基化研究

Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction

摘要:

目的：探讨胰岛素样生长因子结合蛋白3（IGFBP3）启动子区甲基化状态在胎儿宫内生长受限（IUGR）中的作用。方法：选取IUGR新生儿50例及正常新生儿30例，应用甲基化特异性PCR（MSP）及高分辨率溶解（HRM）技术检测外周血中IGFBP3基因的甲基化状态。结果：IUGR组中IGFBP3启动子区完全甲基化比例为4%（2/50），部分甲基化比例为40%（20/50），未甲基化比例为56%（28/50）；对照组中部分甲基化比例为13%（4/30），未甲基化比例为87%（26/30），两组甲基化率差异有统计学意义(P＜0.01)。结论：IGFBP3基因启动子区的甲基化程度与IUGR的发生有关。

Abstract:

OBJECTIVE: To study the role of promoter methylation of insulin-like growth factor binding protein 3 (IGFBP3) in intrauterine growth restriction (IUGR). METHODS: Fifty neonates with IUGR and 30 healthy neonates were enrolled. The promoter methylation status of IGFBP3 in peripheral blood was evaluated by methylation-specific PCR (MSP) and high resolution melting (HRM) techniques. RESULTS: The complete methylation rate, partial methylation rate and non-methylation rate of IGFBP3 promoter in the IUGR group was 4% (2/50), 40% (20/50) and 56% (28/50), respectively. The partial methylation rate and non-methylation rate of IGFBP3 promoter in the control group were 13% (4/30) and 87% (26/30), respectively. There were significant differences in the promoter methylation rate of IGFBP3 between the two groups (P<0.01). CONCLUSIONS: The promoter methylation of IGFBP3 gene is associated with the pathogenesis of IUGR.