黄芪甲甙对急性病毒性心肌炎小鼠IGF-1及相关蛋白表达的作用研究

Effects of astragaloside on IGF-1 and associated protein expression in mice with acute viral myocarditis

摘要:

目的：探讨黄芪甲甙在治疗病毒性心肌炎过程中对胰岛素样生长因子-1（IGF-1）及相关蛋白的影响。方法：65只4周龄Balb/C小鼠随机分为正常对照、黄芪对照、病毒对照及黄芪小剂量、黄芪大剂量等5组。后3组腹腔接种柯萨基B组病毒（CVB3）病毒。黄芪小剂量、黄芪大剂量每日分别给予0.07、0.6 mg/kg黄芪甲甙液灌胃1次。15 d后采血、取心肌组织，用酶标免疫吸附测定法(ELISA)测血浆中IGF-1的表达，用免疫组织化学法检测心肌组织中IGF-1及相关蛋白的蛋白表达，用逆转录聚合酶链反应（RT-PCR）法检测心肌组织IGF-1基因表达。结果：感染CVB3病毒后小鼠心肌组织IGF-1及相关蛋白明显升高，大剂量黄芪甲甙干预可调节小鼠IGF-1及相关蛋白的表达；小剂量黄芪甲甙则无明显效果。结论：大剂量黄芪甲甙对急性病毒性心肌炎小鼠心肌组织的保护作用可能是通过影响IGF-1及相关蛋白表达的途径来实现。

Abstract:

OBJECTIVE: To study the effects of astragaloside on the expression of insulin-like growth factor-1 (IGF-1) and associated proteins in mice with viral myocarditis. METHODS: Sixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg?d, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of IGF-1 in plasma was measured using ELISA. The levels of IGF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction (RT-PCR). RESULTS: The expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of IGF-1 and associated proteins, but low-dose astragaloside did not. CONCLUSIONS: High-dose astragaloside may reduce the expression of IGF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.