抗氧化损伤因子在胎膜早破未足月儿胎盘中的表达及作用

Expression and role of anti-oxidative damage factors in the placenta of preterm infants with premature rupture of membranes

摘要:

目的 探讨抗氧化损伤因子核因子E2相关因子2（nuclear factor erythroid 2-related factor 2，Nrf2）、血红素氧合酶1（heme oxygenase-1，HO-1）及NAD(P)H:醌氧化还原酶1［NAD(P)H:quinone oxidoreductase-1，NQO1］与未足月胎膜早破的相关性。 方法 前瞻性选取2019~2020年在延边医院住院的新生儿为研究对象，其中未足月胎膜早破新生儿30例，足月胎膜早破新生儿32例，足月无胎膜早破新生儿35例。应用苏木精-伊红染色法观察三组胎盘组织炎症变化。采用免疫组织化学染色法检测Nrf2、HO-1及NQO1在胎盘组织中的表达。应用Western blot法检测胎盘组织中Nrf2、HO-1及NQO1的蛋白表达水平。 结果 足月胎膜早破组和足月无胎膜早破组胎盘组织中Nrf2、HO-1及NQO1的阳性表达率及蛋白相对表达量均高于未足月胎膜早破组（P<0.05），足月胎膜早破组胎盘组织中Nrf2、HO-1及NQO1的阳性表达率及蛋白相对表达量与足月无胎膜早破组比较，差异无统计学意义（P>0.05）。 结论 胎盘组织中Nrf2、HO-1及NQO1的低水平表达可能与未足月胎膜早破有关，提示抗氧化损伤是预防未足月胎膜早破的方向之一。

Abstract:

Objective To study the association of the anti-oxidative damage factors nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase-1 (NQO1) with preterm premature rupture of membranes (PPROM). Methods A prospective study was conducted. The neonates who were hospitalized in Yanbian Hospital from 2019 to 2020 were enrolled as subjects, among whom there were 30 infants with PPROM, 32 infants with term premature rupture of membranes (TPROM), and 35 full-term infants without premature rupture of membranes (PROM). Hematoxylin and eosin staining was used to observe the inflammatory changes of placental tissue. Immunohistochemical staining was used to measure the expression of Nrf2, HO-1, and NQO1 in placental tissue. Western blot was used to measure the protein expression levels of Nrf2, HO-1, and NQO1 in placental tissue. Results Compared with the PPROM group, the TPROM group and the non-PROM full-term group had significantly higher positive expression rates and relative protein expression levels of Nrf2, HO-1, and NQO1 in placental tissue (P<0.05). There were no significant differences in the positive expression rates and relative protein expression levels of Nrf2, HO-1, and NQO1 in placental tissue between the TPROM and non-PROM full-term groups (P>0.05). Conclusions The low expression levels of Nrf2, HO-1, and NQO1 in placental tissue may be associated with PPROM, suggesting that anti-oxidative damage is one of the directions to prevent PPROM.