高强度紫外线消毒机器人对多重耐药菌的消毒效果

Disinfection efficacy of high-intensity ultraviolet disinfection robot on multidrug-resistant organisms

摘要:

目的 评估不同条件下，一种自主移动直射式高强度紫外线消毒机器人对环境物体表面多重耐药菌消毒效果。 方法 制备0.5×10⁶ CFU/mL的耐碳青霉烯肺炎克雷伯菌（CRKP）和耐甲氧西林金黄色葡萄球菌（MRSA）染菌载玻片，消毒机器人在不同时间、距离、角度、遮挡及移动状态下对染菌载体进行照射消毒。消毒后使用无菌棉签刮洗染菌载玻片并涂抹至血平板，35℃培养48 h后进行细菌计数，计算不同状态下的杀灭率和杀灭对数值。 结果 静置状态下，距离1 m处，照射5、10 min时，CRKP和MRSA的杀灭率均为100%，杀灭对数值均≥3；距离1 m处，在保鲜膜遮挡和无遮挡条件下，照射5、10 min的CRKP和MRSA杀灭率均为100%，杀灭对数值均≥3，消毒效果达标。移动状态下，对CRKP和MRSA各角度照射时杀灭率均为100%，杀灭对数值均≥3，消毒效果达标。 结论 高强度紫外线消毒机器人可在短时间内对多重耐药菌达到良好的消毒效果，且某些穿透率较高的材质仍维持较佳的消毒结果。

Abstract:

Objective To evaluate the disinfection efficacy of an autonomous mobile direct-radiation high-intensity ultraviolet disinfection robot on multidrug-resistant organisms (MDROs) on environmental object surface. Methods Slides smeared with 0.5×10⁶ CFU/mL carbapenem-resistant Klebsiella pneumoniae (CRKP) and methicillin-resistant Staphylococcus aureus (MRSA) were prepared and disinfected by irradiation of disinfection robot at diffe-rent duration, distances, angles, shielding and moving status. After disinfection, bacterial slides were scraped with sterile cotton swabs and smeared on blood agar plates, plates were then cultured at 35℃ for 48 hours. Bacteria count as well as killing rate and killing logarithm value under different conditions were calculated. Results Under static status, the killing rates of both CRKP and MRSA at an irradiation distance of 1 m for 5 minutes and 10 minutes were 100%, and all killing logarithms were ≥ 3. With irradiation at a distance of 1 m for 5 minutes and 10 minutes respectively, the killing rates of both CRKP and MRSA with and without plastic wrap were 100%, and all killing logarithms were ≥ 3, disinfection efficacy reached the standard. In moving state, the killing rates of CRKP and MRSA at all detected angles were 100%, all killing logarithm value were ≥ 3, and the disinfection efficacy reached standard. Conclusion High-intensity ultraviolet disinfection robot can achieve good disinfection efficacy on MDROs in a short time, the disinfection efficacy remains in case of materials with high penetration rate.