基于数据挖掘分析GPR87在非小细胞肺癌中的表达及临床意义★

Expression status and clinical significance of GPR87 in non-small cell lung cancer: Evidence from data mining★

摘要:

目的 探索G蛋白偶联受体87（GPR87）在非小细胞肺癌（NSCLC）中的表达及意义。方法 通过GE-IA在线工具分析TCGA数据库中GPR87 mRNA在NSCLC和正常肺组织中的表达情况，并进一步分析GPR87基因表达与NSCLC患者TNM分期和总生存期（OS）之间的关系。利用String在线工具分析与GPR87相互作用的蛋白。结果 GPR87 mRNA在肺腺癌（LUAD）和肺鳞癌（LUSC）组织中的表达水平均明显高于正常肺组织（P<0.01）。GPR87 mRNA高表达倾向于与NSCLC患者临床进展期有关，但差异无统计学意义（P>0.05）。TCGA数据库中，GPR87 mRNA高表达组LUAD患者的OS明显短于低表达组（P=0.006 5），并在Kaplan-Meier Plotter数据库中得到了进一步验证（P=0.029）。然而，在TCGA数据库中，GPR87 mRNA高表达组LUSC患者的OS明显长于低表达组（P=0.036），但在Kaplan-Meier Plotter数据库中差异无统计学意义（P=0.35）。String分析显示，LPAR1、LPAR2、LPAR3、SCIN、OR56A3、OR52W1、OR52L1、OR56B4、OR56A1、OR52B2等蛋白与GPR87有明显的相互作用。结论 基于肿瘤基因数据库信息挖掘，GPR87在NSCLC组织中呈高表达，并与LUAD患者预后不良相关，可能是LUAD的潜在分子标志物。

Abstract:

Objective To explore the expression and significance of G protein-coupled receptors 87 (GPR87) in non-small cell lung cancer (NSCLC).Methods The differences in the expressions of GPR87 mRNA between NSCLC and normal lung tissues were compared through The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases by Gene Expression Profiling Interactive Analysis (GEPIA). The relationships of GPR87 gene expression with the TNM stage and overall survival (OS) of patients were further analyzed. Then proteins that interacted with GPR87 were analyzed by using the String database.Results The expression levels of GPR87 mRNA were significantly increased in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues, as compared with that in normal lung tissues (P<0.01). The up-regulation of GPR87 mRNA tended to be associated with the advanced stage of patients, but without statistical significance. In TCGA database, the OS of LUAD patients with high expression of GPR87 mRNA was significantly shorter than that of LUAD patients with low expression of GPR87 mRNA (P=0.006 5), which was further verified in Kaplan-Meier Plotter database (P=0.029). However, in the TCGA database, the OS of LUSC patients with high expression of GPR87 mRNA was significantly longer than that of LUSC patients with low expression of GPR87 mRNA (P=0.036), but such difference was not statistically significant in Kaplan-Meier Plotter database (P=0.35). String database showed that LPAR1, LPAR2, LPAR3, SCIN, OR56A3, OR52W1, OR52L1, OR56B4, OR56A1, OR52B2 had obvious interaction with GRP87.Conclusion Based on the data mining in the gene databases, GPR87 mRNA was highly expressed in NSCLC tissue and closely associated with the poor prognosis of LUAD patients. GPR87 may be a potential molecular marker for LUAD.